Our research group is dedicated to investigating the chemical mechanisms underlying bio-molecular recognition reactions, aiming to simulate natural processes and develop highly selective, sensitive, and efficient analytical methods. Our current research projects encompass:
Molecular Recognition Mechanisms in DNA Damage and Repair Processes:
External chemicals and active metabolites in the body can induce DNA damage. If not promptly repaired, this damage may lead to the development of various diseases, including cancer. Various DNA repair mechanisms within cells ensure the stability of the genome. Our research focus is on understanding how DNA repair enzymes recognize and systematically repair DNA damage, particularly the most frequently occurring base damage. We concentrate on exploring the molecular mechanisms of the Base Excision Repair (BER) pathway, developing efficient fluorescent probes for detecting various BER repair proteins in live cells. Additionally, we have devised methods for the quantitative and precise localization of damage sites.
Precision Molecular Diagnostic Methods for Cancer:
Molecular diagnostics in cancer emphasize the significance of driver genetic alterations. Detecting mutations in circulating cell-free DNA (cfDNA) is challenging due to their typically low levels. We have developed a precise DNA excision tool called sgDNase, utilizing single-stranded phosphorothioated DNA to guide DNase for the removal of wild-type DNA strands at single-nucleotide resolution. The sgDNase assay has been applied to clinical samples, identifying positive samples with low-abundance mutations that are challenging to detect directly using Next-Generation Sequencing (NGS). This provides crucial insights for timely and precise targeted therapy.
Mimicking Bio-molecular Recognition through Molecular Imprinting Technology:
Molecular imprinting is a promising method for generating artificial receptors. Molecularly imprinted polymers (MIPs) are functional materials formed through the co-polymerization of functional monomers and cross-linkers around template molecules. Our more than 20 years of research in molecular imprinting cover fundamental studies and various applications. Recent progress includes the development of biocompatible and structure-controllable protein-imprinted polymers. Surface imprinting on silica substrates has led to hydrogel-silica core-shell particles with rapid kinetics, making them suitable for biosensors and online protein separation. We have also focused on the preparation of dopamine-based magnetic molecularly imprinted nanomaterials, providing powerful tools for studying and regulating changes in the tumor microenvironment and potential development of nanotherapeutic solutions in the future.
